Figure 5
Immunohistochemical analysis of tissue damage evolution after stroke. A) Diagram of the progressive tissue loss after stroke. B) Immunostaining against GAFP (brown) and counterstained with Sirius-red (reddish) to show the glial scar formation with time; C) Immunostaining against CD31 (green), which point an increase in blood vessel; D) Immunostaining against Iba1 (red) to study microglia activation (scale-bar = 50 μm); E) Quantitative analysis of neovascularisation after stroke. F) Scheme of different stages of microglia activation from less activated towards more activated.