IL-8 expression and production by "CF like" like (Solid bars)-and "wild-type like" cells (open bars) upon stimulation with IL-1β and/or TNFα. IL-8 mRNA expression was evaluated by real time-PCR. (A) IL-8 mRNA were significantly higher in AS cells at 3 and 6 h (p = 0.003 and p = 0.0002 respectively) and (B) in IB3-1 cells at 3 h (p = 0.0007). The media were also collected and subject to ELISA to determine IL-8 production. Similarly to the content of IL-8 mRNA, IL-8 protein were significantly high in AS cells at 3 and 6 h (p = 0.004 and p = 0.003 respectively) compared to S cells (A, inset).