Table 1 Transcripts analyzed by real-time PCR, gene symbols and primer sequences
Target transcript | Gene | Polarity | Sequence (5'→3') |
|---|---|---|---|
IDO1 | INDO | Sense | GCATTTTTCAGTGTTCTTCGCATA |
Anti-sense | CATACACCAGACCGTCTGATAGCT | ||
TDO | TDO2 | Sense | GAACATCTTTTTATCATAACTCATCAAGCT |
Anti-sense | ACAACCTTAAGCATGTTCCTTTCAT | ||
KMO | KMO | Sense | TGTAATCCTCCAAGCTTCAATCTG |
Anti-sense | CTAGTAGATGCCCACTGAATATTTGTG | ||
HAAO | HAAO | Sense | GGACGTTCTGTTTGAGAAGTGGTT |
Anti-sense | AGCTGAAGAACTCCTGGATGATG | ||
KAT1 | CCBL1 | Sense | CCTGCTAAGGCTCAGGTATAACCT |
Anti-sense | GGACTCAAGCCTAAAGGCAACTC | ||
KAT2 | AADAT | Sense | CACATCTGGCAGCCAACAAG |
Anti-sense | CACTGGCAACATTAATAATGTTGCA | ||
KAT3 | CCBL2 | Sense | ACTATCAGCCATCCCCGTTTC |
Anti-sense | AATGAAGCAAAAACGCACAAACT | ||
KAT4 | GOT2 | Sense | TGTGGTGTGCAGCCTCTCAT |
Anti-sense | AAGCCTGAACCCAGCTAGCA | ||
KYNU | KYNU | Sense | ACAGGATCTGCCTCCAGTTGA |
Anti-sense | TGGCCCACTTATCTAGTTCTTCTTC | ||
QPRT | QPRT | Sense | ACACCGGCCATGGGTTAAC |
Anti-sense | GCCCCATTGGCCACTGA | ||
GAPDH | GAPDH | Sense | CACATGGCCTCCAAGGAGTAA |
Anti-sense | TGAGGGTCTCTCTCTTCCTCTTGT |