Figure 5From: Loss of function mutation in toll-like receptor-4 does not offer protection against obesity and insulin resistance induced by a diet high in trans fat in miceIn vitro experiments on macrophage cell lines (RAW 264.7 and J774A1). No upregulation of inflammatory markers such as IL-1β (A), IL-6 (B), COX2 (C), or iNOS (C) was observed in macrophages in response to treatment with either elaidic acid or oleic acid at 50 μM and 100 μM concentration level. β-actin was used as a loading control. Abbreviations: COX2, cyclooxygenase 2; iNOS, inducible nitric oxide synthase.Back to article page