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Table 6 Number of total and LECAM+ regulatory T cells and dendritic cells in various hematopoietic and non-hematopoietic tissues in WT vs.

From: Triple selectin knockout (ELP-/-) mice fail to develop OVA-induced acute asthma phenotype

 

Total

     
 

CD45+

CD3+

CD4+

CD8+

CD4+

CD11c+

     

CD25+

 

BM

      

WO

9.67 ± 2.31

0.15 ± 0.06

0.02 ± 0.001

0.12 ± 0.09

0.19 ± 0.06

0.020 ± 0.004

EO

10.16 ± 3.34

0.18 ± 0.07

0.03 ± 0.015

0.14 ± 0.03

0.18 ± 0.07

0.018 ± 0.006

PB

      

WO

26.90 ± 4.41

9.49 ± 4.76

3.59 ± 1.05

5.90 ± 0.87

1.38 ± 0.56

1.18 ± 0.76

EO

29.99 ± 5.67

6.49 ± 2.13

2.22 ± 1.02

4.27 ± 0.56

1.05 ± 0.74

1.61 ± 0.13

Spleen

      

WO

234.89 ± 54.87

118.69 ± 32.87

48.79 ± 11.09

69.90 ± 11.21

27.01 ±

53.91 ± 4.98

EO

212.73 ± 43.98

109.09 ± 18.24

44.76 ± 4.97

63.33 ± 14.32

30.73 ±

41.61 ± 9,61

MLN

      

WO

38.74 ± 4.73

15.22 ± 5.52

4.85 ± 1.09

10.37 ± 2.21

4.95 ± 0.54

7.00 ± 0.43

EO

36.10 ± 9/65

17.48 ± 5.98

4.95 ± 0.67

12.53 ± 3.86

5.30 ± 0.04

7.42 ± 1.09

CLN

      

WO

16.35 ± 3.65

4.41 ± 1.06

1.452 ± 0.04

2.89 ± 0.03

12.91 ± 0.54

2.78 ± 0.54

EO

14.00 ± 4.45

6.36 ± 0.64

5.05 ± 0.43

1.31 ± 0.54

3.51 ± 1.85

2.68 ± 0.31

LNI

      

WO

168.37 ± 7.54

8.67 ± 2.32

2.76 ± 0.54

5.91 ± 1.09

2.82 ± 0.06

3.98 ± 0.43

EO

21.75 ± 3.85

10.53 ± 0.65

2.980.54 ±

7.55 ± 2.03

3.19 ± 1.07

4.47 ± 1.14

LNX

      

WO

11.90 ± 3.28

5.61 ± 0.43

3.93 ± 0.54

3.68 ± 0.54

2.43 ± 0.87

2.26 ± 1.09

EO

13.06 ± 6.90

5.94 ± 1.33

4.71 ± 2.44

2.82 ± 0.98

3.28 ± 0.54

2.50 ± 0.23

LP

      

WO

0.70069 ± 0.01

0.01962 ± 0.0030.004

0.00294 ± 0.003

0.01668 ± 0.003

0.09459 ± 0.003

0.00090 ± 0.00001

EO

0.71086 ± 0.04

0.01987 ±

0.00327 ± 0.001

0.01660 ± 0.004

0.09761 ± 0.004

0.00092 ± 0.00004

  1. KO mice.
  2. Cell suspension was prepared either by flushing or homogenizing tissues such as bone marrow (BM) cells were prepared by flusing femurs, PB (peripheral blood cells were prepared by hemolyzing whole blood to remove RBCs, LP (lung parenchyma) cells were prepared by homogenizing exsanguinated lung tissue and BALf (bronchoalveolar lavage fluid) cells were prepared by flushing airways in the exsangunated lung, spleen cell suspension was prepared by flushing the spleen and MLN (mesenteric lymph node), LNI (Inguinal lymph node), CLN (cervical lymph node) and LNX (axillary lymph node) cells were prepared by similar method. The cells were resuspended in 1X PBS with 5% BSA and stained with fluorochrome conjugated antibodies and quantified by flow cytometry. WO denotes Wild type mice challenged with OVA and EO denotes ELP-/- challenged with OVA. Presented data is average of triplicate of samples (n = 5/group) ± SEM of 2 independent experiments. *p value < 0.01 compared to WT+alum (baseline) values.
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Journal of Inflammation

ISSN: 1476-9255

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