Figure 5
From: Anti-inflammatory function of arctiin by inhibiting COX-2 expression via NF-κB pathways
Arctiin inhibits the expression of co-stimulatory molecules in LPS-stimulated macrophages. RAW 264.7 cells (A-B) were treated with different concentrations of arctiin (12.5~100 μg/ml) in the presence of LPS (100 ng/ml) and was monitored as described in 'Materials and Methods'. The surface B7-1 (A) and B7-2 (B) molecules were labeled with either anti-B7-1, B7-2 and the cell were stained using anti-Vβ8.1+8.2-FITC, anti-Vβ2-PE, anti-Vβ2-FITC (shaded histogram), which served as an isotype control for the nonspecific binding.