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Table 2 T-bet KO mice develop less severe alveolitis following long-term exposure to S. rectivirgula compared to WT mice

From: T-bet controls severity of hypersensitivity pneumonitis

 

Alveolitisa

PMNb(%+/-SD)

CD4 T cellsb(%+/-SD)

CD8 T cellsb(% +/- SD)

NK cellsb(% +/- SD)

 

WT

Tbet-/-

WT

Tbet-/-

WT

Tbet-/-

WT

Tbet-/-

WT

Tbet-/-

3 wk SR

2.2 × 106+/-1.2

1.9 × 106+/-1.9

72+/-6.0

70+/-14

6+/-1.5

9+/-2.5

3+/-0.7

3+/-1.1

5+/-1.3

2+/-0.1

6 wk SR

10.3 × 106+/-2.5

3.1 × 106 +/-2.2*

88+/-3.0

91+/-1

4+/-0.4

3+/-1.0

2+/-0.3

1+/-0.4

<1

<1

  1. C57BL/6 or T bet-/- mice (n = 5/group) were exposed to S. rectivirgula (150 μg) three times a week for three or six weeks.
  2. a BAL was performed and the recovered cells were counted using trypan blue dye exclusion.
  3. b Cells isolated from the BAL fluid were incubated with antibodies to various cell surface markers and analyzed by flow cytometry to determine the cellular composition of the BALF. CD4+ T cells were identified as βTcR+/CD45+/CD4+; CD8+ T cells - βTcR+/CD45+/CD8+; PMNs - CD45+/CD11b+/Gr1+; NK cells - CD45+/NK1.1+
  4. *p < 0.05 compared to WT/S. rectivirgula group
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Journal of Inflammation

ISSN: 1476-9255

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