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Table 2 T-bet KO mice develop less severe alveolitis following long-term exposure to S. rectivirgula compared to WT mice

From: T-bet controls severity of hypersensitivity pneumonitis

  Alveolitisa PMNb(%+/-SD) CD4 T cellsb(%+/-SD) CD8 T cellsb(% +/- SD) NK cellsb(% +/- SD)
  WT Tbet-/- WT Tbet-/- WT Tbet-/- WT Tbet-/- WT Tbet-/-
3 wk SR 2.2 × 106+/-1.2 1.9 × 106+/-1.9 72+/-6.0 70+/-14 6+/-1.5 9+/-2.5 3+/-0.7 3+/-1.1 5+/-1.3 2+/-0.1
6 wk SR 10.3 × 106+/-2.5 3.1 × 106 +/-2.2* 88+/-3.0 91+/-1 4+/-0.4 3+/-1.0 2+/-0.3 1+/-0.4 <1 <1
  1. C57BL/6 or T bet-/- mice (n = 5/group) were exposed to S. rectivirgula (150 μg) three times a week for three or six weeks.
  2. a BAL was performed and the recovered cells were counted using trypan blue dye exclusion.
  3. b Cells isolated from the BAL fluid were incubated with antibodies to various cell surface markers and analyzed by flow cytometry to determine the cellular composition of the BALF. CD4+ T cells were identified as βTcR+/CD45+/CD4+; CD8+ T cells - βTcR+/CD45+/CD8+; PMNs - CD45+/CD11b+/Gr1+; NK cells - CD45+/NK1.1+
  4. *p < 0.05 compared to WT/S. rectivirgula group