T-bet controls severity of hypersensitivity pneumonitis

Journal of Inflammation

Table 1 T-bet^-/- mice develop less severe alveolitis compared to WT and IL-18^-/^- mice following exposure to S. Rectivirgula

	Alveolitis^a	%PMN^b	Total PMN^b(% +/- SD)	Total NK^b(+/-SD)
WT/unexp	0.09 × 10⁶+/-0.04	-	-	-
WT/ *S. rectivirgula*	1.5 × 10⁶+/-0.8	73+/-5	1.1 × 10⁶+/-0.5	2.03 × 10⁴+/-0.7
Tbet ^-/- / *S. rectivirgula*	0.5 × 10⁶+/-0.3 *	66+/-10	0.4 × 10⁶+/-0.2 *	0.05 × 10⁴+/-0.07 *
IL-18 ^-/- / *S. rectivirgula*	1.3 × 10⁶+/-0.6	78+/-3	1.0 × 10⁶+/-0.5	4.1 × 10⁴+/-2.0

C57BL/6, IL-18^-/- or T bet^-/- mice (n = 5/group) were exposed to S. rectivirgula (150 μg) three times and analyzed 18 hrs after last exposure.
^a BAL was performed and the recovered cells were counted using trypan blue dye exclusion.
^b Cells isolated from the BAL fluid were incubated with antibodies to various cell surface markers and analyzed by flow cytometry to determine the cellular composition of the BALF. PMNs were identified as: CD45⁺/CD11b⁺/Gr1⁺; NK cells as CD45⁺/NK1.1⁺
* p < 0.05 compared to WT/S. rectivirgula group.

ISSN: 1476-9255