IFNγ and IL-17 mRNA are decreased in T-bet-/-and IL-18R-/-mice following S. rectivirgula exposure. C57BL/6, IL-18-/-, or T-bet-/- mice (5/group) were intranasally exposed to S. rectivirgula (150 μg) or saline for 3 days. A.) RNA was isolated from lungs of individual mice, reverse transcribed, and real-time PCR performed using primers specific for MIP-2, IFNγ and IL-17. The results were normalized to the housekeeping gene HPRT and expressed as fold induction over unexposed mice. B.) IFNγ was measured in the BALF of individual mice by ELISA.