Figure 4

Leishmania promotes the production of LPS-induced MIP-1α and MCP-1. Cells were incubated with 100 ng/ml LPS followed by the addition of L. major, L. amazonensis or latex beads at a parasite/bead: host cell ratio of 20:1 (indicated by blue, green and open bars, respectively). At 2 hr, 8 hr and 19 hr timepoints, 200 μl of culture supernatant was removed, centrifuged then assayed for cytokine expression by Bio-Plex assay as described in Methods. Media only, L. major alone, L. amazonensis alone, latex beads alone and LPS alone (black, gray, purple, brown and red bars, respectively) were used as controls. Data represented are averages of two independent replicate experiments, with error bars as indicated.