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Table 2 Effect of colitic inducers, TNBS and DSS, on number of anaerobes and Enterobacteriaceae in C3H/HeN and C3H/HeJ mice

From: Dextran sulfate sodium and 2,4,6-trinitrobenzene sulfonic acid induce lipid peroxidation by the proliferation of intestinal gram-negative bacteria in mice

Mouse

Colitic inducer

Number of colonies grown in agar plate

  

GAM (Anaerobes)(×1010)

BL (Bifido)(×108)

DHL (×106)

    

Ec

Pm

Kp

C3H/HeN

-a

3.3 ± 0.5

6.6 ± 0.5

0.9 ± 0.3

0.9 ± 0.3

0.5 ± 0.2

 

DSS

2.3 ± 0.7

3.9 ± 0.5#

5.1 ± 2.0#

2.8 ± 0.6#

14.0 ± 2.5#

C3H/HeJ

-a

1.0 ± 0.4

4.2 ± 0.3

0.9 ± 0.2

-b

5.0 ± 0.9

 

DSS

1.6 ± 0.5

3.7 ± 1.1#

4.7 ± 0.6#

-b

9.2 ± 0.9#

C3H/HeN

-a

3.2 ± 0.7

5.9 ± 1.1

0.3 ± 0.04

0.9 0.1

0.7 0.1

 

TNBS

1.9 ± 0.8#

0.5 ± 0.1#

3.2 ± 0.3#

1.8 ± 0.1#

17.8 ± 2.0#

C3H/HeJ

-a

1.7 ± 0.6

20.9 ± 2.1

3.5 ± 0.6

-b

2.8 ± 0.2

 

TNBS

0.6 ± 0.1#

7.0 ± 0.8#

49.1 ± 4.7#

-b

31.8 ± 8.6#

  1. The test agents were orally administered for 3 days prior to TNBS treatment. The fresh feces was plated in BL, GAM, and DHL agar plates and cultured anaerobically (for BL and GAM agar plates) or aerobically (for DHL agar plates). The colonies grown in DHL agar media were selected and identified by 16S rDNA analysis. Bifido, bifidobacteria; Ec, Escherichia coli; Pm, Proteus mirabilis; Kp, Klebsiella pneumonia.
  2. a) Normal group treated with vehicle alone instead of colitic inducer.
  3. b) Not detected.
  4. All values are the mean ± S.D. (n = 10). #Significantly different vs. normal group in each column of C3H/HeN or C3H/HeJ mice (P < 0.05).