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Figure 4 | Journal of Inflammation

Figure 4

From: Dextran sulfate sodium and 2,4,6-trinitrobenzene sulfonic acid induce lipid peroxidation by the proliferation of intestinal gram-negative bacteria in mice

Figure 4

Intestinal bacteria increase lipid peroxidation in liposomes prepared with L-α-phosphatidylcholine. Lipid peroxide in liposome was estimated by thiobarbituric acid-reactive substance assay. The intestinal bacteria previously cultured in GAM broth were collected by centrifugation (10,000 × g for 30 min) and washed twice with PBS. The resulting pellet was suspended in phosphate-buffered saline: Ba, Bifidobacterium animalis; Bc, Bifidobacterium cholerium; Ec, Escherichia coli; Lp, Klebsiella pneumonia e; Pm, Proteus mirabilis. Bh indicates butylated hydroxyl-anisole. Test agents [20 (white bar), and 50 mg/ml (black bar)] was treated. All values are the mean ± S.D. (n = 3).

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