TMP inhibits RelA DNA binding. RAW 264.7 cells were either stimulated with 1 μg/ml of LPS or 1 μg/ml of LPS and 25 μM TMP for 4 hours. Following treatment, protein:DNA complexes were cross-linked, and RelA binding at the TNF-α (A), MCP-1/CCL2 (B) and RANTES/CCL5 (C) promoters was assessed by chromatin immunoprecipitation. Enrichment was calculated relative to pre-IP input control levels and was normalized to signals obtained with non-specific IgG control antibodies. Data shown are representative of two independent experiments and chromatin preparations. Asterisks indicate significant differences between LPS treatments and LPS treatments with TMP (p < 0.05, T-test).