Mouse cytokine protein array of SCp2 cells following treatment with ET. Subconfluent SCp2 cells were treated with ET (0 or 10 μg/ml) in 1% FBS DM for 1, 3, 6, and 12 h, and medium collected at each time point was assayed for different inflammatory cytokines (A) by the RayBio Mouse Cytokine Antibody Array I. (B) Layout of the mouse cytokine protein array on the membrane. Abbreviations POS = positive control, NEG = negative control. GCSF = granulocyte colony stimulating factor, GM-CSF = granulocyte macrophage colony stimulating factor, IL = interleukin, IFN-γ = interferon gamma, MCP = monocyte chemotactic protein, RANTES = regulated on activation, normal T expressed and secreted, SCF = stem cell factor, sTNFR = soluble tumor necrosis factor α receptor, TNFα = tumor necrosis factor alpha, VEGF-vascular endothelial growth factor. Densitometric analysis of (C) IL-6 and (D) GCSF proteins secreted in response to ET at different time points was calculated using NIH Image J program. Error bars denote the standard deviation (SD) between duplicate of a representative dot blot.