Figure 7

Effect of SCg6 monolayer on basal and ET-induced IL-6 secretion and NO production in SCp2 cells. SCg6 and SCp2 cells were cocultured as described in Methods. The medium was collected 24 h later and assayed for basal (open bar) or ET-induced (closed bar) (A) IL-6 secretion and (C) NO production. (B) represents basal and ET-induced IL-6 secretion in SCg6, SCp2 and SCp2:SCg6 coculture normalized to cell number. Data represents the average for IL-6 protein or nitrite concentrations from 2 independent experiments ± SEM. (*) denotes significant differences between treatment within each time point (p < 0.05) and letters denote significant differences among cell types within treatment (p < 0.05).