ET induces binding activity of NFκB p65 and p50 in SCp2 cells. SCp2 cells were treated as described in Methods with 0 or 10 μg of ET per ml in 1% FBS-DM for 0, 1, 3, and 6 h. Isolated nuclear proteins of control (0 μg ET) and ET-treated (10 μg ET) SCp2 cells were analyzed for (A) NFκB p65 or (B) NFB p50 binding activities by immunobinding assays, with relative binding activity shown as A690 above blank. Solid-line and closed symbol represent the results of control non ET-treated samples, while dashed-line and open symbol represent the results of samples from ET-treated cells. This experiment was performed at least twice. Data represents the average for duplicate samples ± SD of a representative experiment. (*) denotes significant difference among treatment within each time point (p < 0.05).