Figure 2
From: HE3286, an oral synthetic steroid, treats lung inflammation in mice without immune suppression
Effect of HE3286 treatment on MPO levels in LPS induced Lung Injury. On day-1, male C57 black/6 mice were pre-treated (gavage) with HE3286 or 0.1 mL vehicle (HERF405). The next day, mice were challenged with 50 μg of E-coli LPS under direct visualization of trachea under light anesthesia. Sixty minutes after the LPS challenge, mice were treated with a second dose of HE3286, or vehicle. Forty-eight hours after LPS challenge, mice were sacrificed and myeloperoxidase (MPO) activity in lungs determined as previously described [45]. Results are from two identical experiments. Data are expressed as O.D at 460 nM.