Figure 7From: Defining human mesenchymal stem cell efficacy in vivoLung Inflammation Determines Response to hMSCs. BAL fluid was obtained from mice treated with hMSCs just prior to challenge and initiation of inflammation (pre-) or 3 days after initiation of inflammation (post-). BAL differentials were obtained from each animal using cytospins and Wright-Giemsa stain. Neutrophil counts are in panel A, lymphocytes in panel B, monocytes in panel C and eosinophils in panel D. The first bar represents the differential in the model without hMSCs. No difference was observed in levels of neutrophils or lymphocytes after hMSC treatment. Pre-challenged mice had a decrease in eosinophils (panel D, n = 4, p = 0.05) compared to control mice. This decrease in eosinophils was amplified at post-treatment with hMSCs. Additionally, post-treatment mice had a significant increase in localized levels of monocyte/macrophages (panel C, n = 2, p = 0.05, 4 animals used in each group for each experiment).Back to article page