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Table 5 Effect of tuberosin on LPS induced NO production and iNOS expression by attached rat peritoneal macrophages.

From: Antioxidant activity of tuberosin isolated from Pueraria tuberose Linn

Concentration of tuberosin (ng/ml)

NO production (μg/104cells)

Pixel value of iNOS bands in western blot

Normal Cells

10.11 ± 1.043

-

   Only LPS (20 ng/ml)

39.89 ± 1.983

16023

LPS(20 ng/ml)+Tuberosin(ng/ml)

 

-

100

38.09 ± 1.933

15878

200

36.09 ± 1.862**

-

300

27.02 ± 1.698**

10678

400

21.16 ± 1.829*

-

500

13.04 ± 1.904*

-

600

10.09 ± 1.898*

5082

LPS + Quercetin(50 ng/ml)

9.98 ± 1.041

4223

EC50 of tuberosin

399.68 ng/ml

-

EC50 of quercetin

190 ng/ml

-

  1. Values were significant (p* < 0.1, p** < 0.001) when compared with experimental control.