Table 4 Effect of tuberosin in the deoxyribose assay in the presence of EDTA (non-site specific) to assess the Hydroxyl radical scavenging activity and absence of EDTA (site specific) to assess metal chelation property
From: Antioxidant activity of tuberosin isolated from Pueraria tuberose Linn
Concentration of tuberosin (mM) | Absorbance at 532 nm (mean ± S.D) | % decrease in absorbance (mean ± SD) | ||
|---|---|---|---|---|
| Â | (Non site specific) | (Site specific) | (Non site specific) | (Site specific) |
Normal | 0.310 ± .018 | 0.515 ± .028 | -- | -- |
Blank | 0.288 ± .017 | 0.485 ± .022 | -- | -- |
0.25 | 0.280 ± 0.014* | 0.400 ± .024* | 2.60 ± 0.94 | 17.54 ± 1.25 |
0.50 | 0.256 ± .015* | 0.356 ± .021** | 11.22 ± 0.97 | 26.54 ± 1.15 |
0.75 | 0.235 ± .013** | 0.311 ±.020** | 18.35 ± 0.88 | 35.82 ± 0.91 |
1.00 | 0.199 ± .012** | 0.271 ± .018** | 30.73 ± 1.08 | 44.08 ± 0.96 |
1.25 | 0.156 ± .011** | 0.190 ± .011** | 45.94 ± 0.94 | 60.80 ± 0.94 |
1.80 | 0.125 ± .010** | 0.119 ± .008** | 56.72 ± 1.14 | 75.51 ± 0.99 |
2.50 | 0.092 ± .007** | 0.01 ± .001** | 67.94 ± 0.67 | 98.04 ± 0.62 |