Figure 5
Inhibition of T-cell proliferation responses in MLRs by Didox and Trimidox. (A) Major antigen mismatch: Briefly, 2 × 105 T-cells were purified from spleens C57BL6 mice (responder cells) were mixed with 4 × 105 irradiated non T-cells (stimulator cells) obtained from BALB/c mice (exposed to 3000 rads of γ-radiation). (B) Minor antigen mismatch: Briefly, 2 × 105 T-cells were purified from spleens B10.D2 mice (responder cells) which were mixed with 4 × 105 non T-cells (stimulator cells) obtained from BALB/c and pre-exposed to 3000 rads of γ-radiation. Either PBS (untreated) or several concentrations of Didox or Trimidox (25 μM- 100 μM) was added to RPMI 1640 growth media supplemented with 10% fetal bovine serum, 50 μM 2-Mercaptoethanol and 1% penicillin streptomycin. The plates were then incubated at 37°C and 5% CO2 for 6 days, after which spectrophotometric quantification of cell growth and proliferation was determined. The results shown represent data obtained in triplicate from two independent experiments. Values shown represent the mean ± SD obtained in triplicate from two independent experiments. * indicates a significant difference compared to PBS treated (Untreated). (p < 0.05, ANOVA + the Bonferroni test).