Cellular toxicity of Didox and Trimidox in T-cells from C57BL6 and B10.D2 mouse strains. Briefly, 1 × 105 cells per well were seeded in 96 well culture plates in RPMI 1640 supplemented with 10% fetal bovine serum and 1% penicillin streptomycin and 2-mercaptoethanol, containing PBS or concentrations of Didox or Trimidox from 25 μM- 100 μM. The cells were then incubated at 37°C and 5% CO2 for 4 days. After this, percentage of viable cells of (A) Didox and (B) Trimidox was determined for each drug dose. * indicates a significant difference compared to PBS treated (UT). (p < 0.05, ANOVA + the Bonferroni test; n = 3).