Figure 5

JNK pathway is activated by S. typhimurium with or without TNF-α pretreatment. A. The expression level of proteins associated with the JNK pathway in intestinal epithelial cells colonized with Salmonella. Intestinal epithelial cells were incubated with an equal number of the indicated S. typhimurium with or without TNF-α pretreatment. Epithelial cells were collected. Cell lysates were immunoblotted with the indicated antibodies. β-actin is an internal control for the Western blot. The data are reported as the mean ± SD of three independent experiments. (B) (C) Relative intensity of Western blot bands. Salmonella exposed to TNF-α induced higher activity of JNK pathway with enhanced p-JNK and p-c-Jun in the host cells. The data are reported as mean ± SD of three independent experiments. *P < 0.05 was considered significant. (D). The expression level of proteins associated with the JNK pathway in intestinal epithelial cells colonized with Salmonella. HCT116 cells were pretreated with a JNK inhibitor SP600125 (50 μM).