The inflammatory response seen when human omental adipose tissue explants are incubated in primary culture is not dependent upon albumin and is primarily in the nonfat cells

Table 1 Effect of albumin on the changes in gene expression over a 2 h incubation

mRNA	Fold change over 2 h	% change in the presence of 1% albumin
IL-8	87 ± 8***	+540 ± 44%***
IL-1β	24 ± 4***	+6000 ± 60%***
TNFα	6.1 ± 0.9***	+920 ± 50%***
HIF-1α	2.4 ± 0.8**	-10 ± 20%
NFKB₁ [p50]	3.5 ± 0.9**	+40 ± 18%
Omentin/intelectin	0 ± 1	0 ± 20%

Explants of human omental adipose tissue were incubated for 2 h in buffer without or with 1% albumin. The basal data are shown as the mean ± SEM for eight experiments of the ratio of each mRNA at 2 h to that at the start of the incubation. The effects of the albumin are the mean ± SEM of the paired percentage differences. Significant effects of the 2-h incubation and of albumin or serum are indicated as follows: * P < 0.05, ** P < 0.025 and *** P < 0.01

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