Table 1 Cocktail compositions and the cocktail stimulated DC expression pattern for selected cytokines and chemokines on cytokine arrays
Cocktail | Composition | Secreted cytokines and chemokines | ||
|---|---|---|---|---|
Weak induction (1-10 fold) | Modest induction (10-100 fold) | Strong induction (> 100 fold) | ||
LPS | LPS (0,1ug/mL) | IL-8, CCL2, CCL4, CXCL5, CXCL10, GM-CSF | IL-10, CCL20, CXCL1-3 | IL-6, IL-12, TNFα, VEGF, CCL8, CCL15, CXCL1 |
Cocktail 1 | IFNγ (20ng/mL)+ TNFα (50ng/mL)+ Poly I:C 12,5 ug/mL + IL-1β (10ng/mL) + IFNα (6ng/mL) | IL-8, CCL2, CCL4, CCL5, CXCL5, CXCL10 | IL-10, CCL20, CXCL1-3, GM-CSF | IL-6, IL-12, TNFα, VEGF, CCL8, CCL15, CXCL1 |
Cocktail 2 | IFNγ (20ng/mL)+ Poly I:C 12,5 ug/mL + IL-1β (10 ng/mL) + IFNα (6ng/mL) | IL-8, CCL2, CCL4, CCL5, CXCL5, CXCL10 | IL-10, CCL20, CXCL1-3, GM-CSF | IL-6, IL-12, TNFα, VEGF, CCL8, CCL15, CXCL1 |
Cocktail 3 | IFNγ (20ng/mL)+TNFα (50ng/mL)+ peptidoglycan (10ug/mL) | IL-8, CCL2, CCL4, CCL5, CXCL5 | IL-10, CCL20, CXCL1-3, CXCL10, GM-CSF | IL-6, IL-12, TNFα, VEGF, CCL8, CCL15, CXCL1 |
Cocktail 4 | LPS (0,1ug/mL)+ IFNγ (20ng/mL) | IL-8, CCL2, CCL4, CCL5, CXCL5, GM-CSF | IL-10, CCL20, CXCL1-3, CXCL10 | IL-6, IL-12, TNFα, VEGF, CCL8, CCL15, CXCL1 |
Cocktail 5 | LPS (0,1ug/mL)+ IFNγ (20ng/mL)+ TNFα (50ng/mL) | IL-8, CCL2, CCL4, CCL5, CXCL5, GM-CSF | IL-10, CCL20, CXCL1-3, CXCL10 | IL-6, IL-12, TNFα, VEGF, CCL8, CCL15, CXCL1 |
Cocktail 6 | R848 (1,0ug/mL) | IL-8, CCL2, CCL4, CCL5, CXCL10 | IL-10, CCL15, CCL20, CXCL1-3, CXCL5, GM-CSF | IL-6, IL-12, TNFα, VEGF, CCL8, CXCL1 |
Cocktail 7 | R848 (1,0ug/mL) + IFNγ (20ng/mL) | IL-8, CCL2, CCL4, CCL5, CCL20, CXCL5, GM-CSF | IL-10, CCL15, CCL20, CXCL1-3, CXCL10 | IL-6, IL-12, TNFα, VEGF, CCL8, CXCL1 |
Cocktail 8 | R848 (1,0ug/mL) + poly I:C (10ug/mL) | IL-8, CCL2, CCL4, CCL5, CXCL5, CXCL10 | IL-10, CCL20, CXCL1-3, GM-CSF | IL-6, IL-12, TNFα, VEGF, CCL8, CCL15, CXCL1 |
Cocktail 9 | IFNγ (20ng/mL) + IL-1β (10ng/mL) | IL-8, CCL2, CCL4, CCL5, CXCL5, GM-CSF | IL-10, CCL15, CCL20, CXCL1-3, CXCL10 | IL-6, IL-12, TNFα, VEGF, CCL8, CXCL1 |