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Figure 5 | Journal of Inflammation

Figure 5

From: Differential induction of inflammatory cytokines by dendritic cells treated with novel TLR-agonist and cytokine based cocktails: targeting dendritic cells in autoimmunity

Figure 5

FACS profile on cocktail stimulated DCs. Surface staining by flow cytometric analysis of immature (untreated), LPS and cocktail stimulated DCs. The surface expression of relevant activation markers was analyzed on day 7. A) A total of 5000 events were collected by gating hDCs defined by forward (FSC) and side-scatter (SSC) characteristics. All histograms were gated for CD1a cells (70-95%). All our cells were CD14 negative (data not shown). Flow cytomeric analysis of maturation markes were done for DCs from three donors stimulated with LPS or cocktails, and results normalized to the untreated DCs (average value of Mean florescence intensity for untreated DCs from the three donors was set to 100%). The vertical bars indicate standard deviation (SD) values. B) Phenotypic surface analysis of the suppressive effect of dex on cocktail treated human DCs from two donors. Pre-treatment of immature DCs with dex for 6 h before addition of selected cocktails reduced the expression of activation markers. Results for the different treatments have been normalized in proportion to the untreated DC. The vertical bars indicate (SD) values.

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