Figure 3

Dexamethasone prevents cocktail induction of IL-12p70 and TNFα. Immature DCs from a single donor shows a dexamethasone-mediated dose dependent suppression of IL-12p70 and TNFα secretion. Dexamethasone was pre-incubated with imDCs for 6 hours (A and C) or 24 hours (B and D) with increasing concentration of dexamethasone at 0-0.01-0.1 and 1.0 μM. Dexamethasone treatment without cocktail did not induce IL-12p70 or TNFα (first 4 bars). The cocktails used are indicated below each set of data, and their exact composition is seen in table 1. This shows one representative example out of three. Cell viability was not significantly affected by treatment with cocktail and/or dexamethasone (not shown).