Figure 2

Cytokine array using conditioned media from cocktail treated DCs. Cytokine array membranes were incubated with conditioned media from DCs treated with cocktails for 24 h. To compensate for donor variations the conditioned media from four donors was mixed. Upper left four spots and lower right two spots serves as positive controls on each membrane. On the figure constitutively secreted cytokines can be seen on the picture indicated Neg. ctrl, which were untreated cells. Each induced protein is marked only once, squares mark DC-produced cytokines and chemokines, and a circle marks a cytokine which is added as part of the cocktail (TNFα, IL-1β and IFNγ).