Figure 5

Effect of DFP on PMN oxidative burst activity and hydrogen peroxide production. Human neutrophils were incubated alone (Control), with LPS (1 μg/mL) or with DFP (2.5 mM) for 2 hours. Cells were then incubated with 1 μM of DHR followed by incubation with 10^-7 M fMLP. Cells were analyzed by flow cytometry to detect the conversion of DHR 123 to rhodamine 123. A. Mean fluorescence values are shown for a minimum of 10 000 cells for each condition and are representative of 9 separate experiments. B. Oxidative burst activity of neutrophils treated with or without LPS or DFP (2.5 mM). Data represent mean ± SD of 9 separate experiments. *P < 0.002. C. Neutrophils were incubated alone (control), with LPS (1 μg/mL) or with DFP (2.5 mM) for 0, 1 and 2 hours. 2 × 10⁴ cells were incubated with Amplex Red reaction mixture and 10^-7 M fMLP. Hydrogen peroxide production was measured using fluorimetric reader, and expressed in μM. Data represent mean ± SD of 7 separate experiments. *P = 0.015