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Figure 3 | Journal of Inflammation

Figure 3

From: Regulation of apoptosis and priming of neutrophil oxidative burst by diisopropyl fluorophosphate

Figure 3

Effect of DFP on processing of pro-caspases-3 and -8 and caspases-3 and -8 activity. Human neutrophils were incubated alone (Control) or with DFP 2.5 mM for 5 hours. Cells were then lyzed and lysates were separated on 12% SDS-PAGE gel and specific antibodies were used to evaluate the pattern of caspase-8 (A) and caspase-3 (B) processing. Blot is representative of 3 separate experiments. C. Human neutrophils were incubated alone (Control), with LPS (1 μg/mL) or with DFP (2.5 mM) for 5 hours. Cells were then lyzed. Caspase-8 and caspase-3 activities were measured using specific colorimetric and fluorimetric substrates respectively. Caspase-8 (C) activity is represented as absorbance at 405 nm & caspase-3 (D) activity is represented as fluorescence units at excitation wavelength of 360 nm and emission wavelength of 460 nm. Data represent mean ± SD of 4 separate experiments. *P = 0.243 for caspase-8; *P = 0.02 for caspase-3.

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