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Figure 2 | Journal of Inflammation

Figure 2

From: Regulation of apoptosis and priming of neutrophil oxidative burst by diisopropyl fluorophosphate

Figure 2

Effect of DFP on neutrophil constitutive apoptosis. Human neutrophils were incubated alone (Control), with LPS (1 μg/mL) or with increasing doses of DFP for 20 hours. Cells were permeabilized with Triton X-100 and then stained with propidium iodide (50 μg/mL). A. Mean fluorescence values are shown for a minimum of 10 000 cells for each condition and are representative of 3 determinations from 13 separate experiments. B. Rate of apoptosis (hypodiploid DNA) of neutrophils treated with or without LPS or DFP (2.5 mM) are represented. Data represent mean ± SD of 15 separate experiments. *P < 0.05. C. Rate of apoptosis (hypodiploid DNA) of neutrophils treated with or without increasing doses of DFP. Data represent mean ± SD of 2 to 8 separate experiments. D. Rate of apoptosis (hypodiploid DNA) of neutrophils treated with LPS or DFP (2.5 mM) from control cells cultured in serum free medium. Results are mean ± SD of 2 separate experiments.

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