Figure 1From: Regulation of apoptosis and priming of neutrophil oxidative burst by diisopropyl fluorophosphateEffect of DFP on NE activity and neutrophil degranulation. Human neutrophils were incubated in medium alone (Control), with LPS (1 μg/mL) or with DFP at increasing doses (25 nM, 2.5 μM, 250 μM, and 2.5 mM) for 5 hours. Cells were then lyzed and NE activity was measured at an excitation wavelength of 360 nm and emission wavelength of 460 nm; results are represented as arbitrary fluorescence units. A. NE activity of neutrophils treated with or without LPS or DFP (2.5 mM). Data represent the mean ± SD of 9 separate experiments. *P = 0.010. B. NE activity of neutrophils treated with increasing doses of DFP; results are from a single experiment. C. Human neutrophils were incubated with or without DFP (2.5 mM) for 1 hour and peroxidase release was measured as described in Materials and Methods. Peroxidase release was expressed as percentage of the total peroxidase activity of 150 000 neutrophils treated with 0.02% CTAB. Data represent mean ± SD of 4 separate experiments. P = NS.Back to article page