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Figure 1 | Journal of Inflammation

Figure 1

From: Regulation of apoptosis and priming of neutrophil oxidative burst by diisopropyl fluorophosphate

Figure 1

Effect of DFP on NE activity and neutrophil degranulation. Human neutrophils were incubated in medium alone (Control), with LPS (1 μg/mL) or with DFP at increasing doses (25 nM, 2.5 μM, 250 μM, and 2.5 mM) for 5 hours. Cells were then lyzed and NE activity was measured at an excitation wavelength of 360 nm and emission wavelength of 460 nm; results are represented as arbitrary fluorescence units. A. NE activity of neutrophils treated with or without LPS or DFP (2.5 mM). Data represent the mean ± SD of 9 separate experiments. *P = 0.010. B. NE activity of neutrophils treated with increasing doses of DFP; results are from a single experiment. C. Human neutrophils were incubated with or without DFP (2.5 mM) for 1 hour and peroxidase release was measured as described in Materials and Methods. Peroxidase release was expressed as percentage of the total peroxidase activity of 150 000 neutrophils treated with 0.02% CTAB. Data represent mean ± SD of 4 separate experiments. P = NS.

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