Figure 6

Fas-induced release of HMGB1 by primary murine peritoneal mϕ is TLR4/IRAK4-dependent. Primary murine peritoneal mϕ derived from (A) wild-type vs Tlr2^-/-vs Tlr4^-/-mice, or (B) wild-type vs Irak4^-/-mice, were stimulated with mFasL (0.25 μg/ml) for the times indicated (0 - 6 hr) as described in Materials and Methods. HMGB1 release into the concentrated cell culture-conditioned medium was detected by immunoblot as described in Materials and Methods. Results are representative of 3 independent experiments that yielded similar results.