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Figure 2 | Journal of Inflammation

Figure 2

From: Fas (CD95) induces rapid, TLR4/IRAK4-dependent release of pro-inflammatory HMGB1 from macrophages

Figure 2

Fas activation for 24 hr does not affect viability of primary murine peritoneal mϕ. (A) Dose response analysis of viability of primary murine peritoneal mϕ treated with recombinant mFasL (0-0.5 μg/ml); (B) time course (0 - 24 hr) analysis of primary peritoneal mϕ following treatment with recombinant mFasL (0.5 μg/ml). Cell viability was determined by trypan blue exclusion using a cell viability analyzer as described in Materials and Methods. Results are presented as mean ± SD of 3 independent experiments, n = 3/group. * P ≤ 0.05; **P ≤ 0.01.

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