Fas activation for 24 hr does not affect viability of primary murine peritoneal mϕ. (A) Dose response analysis of viability of primary murine peritoneal mϕ treated with recombinant mFasL (0-0.5 μg/ml); (B) time course (0 - 24 hr) analysis of primary peritoneal mϕ following treatment with recombinant mFasL (0.5 μg/ml). Cell viability was determined by trypan blue exclusion using a cell viability analyzer as described in Materials and Methods. Results are presented as mean ± SD of 3 independent experiments, n = 3/group. * P ≤ 0.05; **P ≤ 0.01.