Figure 1

Fas activation induces rapid release of HMGB1 from primary murine peritoneal mϕ and RAW264.7 cells . (A) HMGB1 in cell culture-conditioned medium was detected by immunoblot as described in Materials and Methods following treatment with recombinant mFasL (0 - 0.5 μg/ml) for 2 hr. (B) HMGB1 in cell culture-conditioned medium was detected by immunoblot as described in Materials and Methods following treatment with mFasL, mAb Jo2 and LPS respectively (0.25 μg/ml for each stimulus) for the times indicated (0 - 24 hr). (C) HMGB1 in cell culture-conditioned media from primary wild-type and Fas^lprmurine peritoneal mϕs culture-conditioned medium was detected by immunoblot as described in Materials and Methods following treatment with mFasL, mAb Jo2 and LPS (0.25 μg/ml for each stimulus), respectively, for the times indicated (0 - 24 hr). All the results are representative of 3 independent experiments that yielded similar results.