Figure 1
Effect of AS101 on LPS-induced iNOS protein expression (A-D) and NO(E) and IL-6 (F) secretion. (A) LPS-stimulated RAW264.7 cells (1 × 106/ml) were treated with AS101(0.5 or 2 [μg/ml]) for 1 h (A) and 4 h (C). The iNOS level was analyzed by immunoblotting using anti-iNOS. Actin was used as an internal loading control. Bar graphs represent the quantitative densitometric value of the expressed protein vs actin: 1 h (B) and 4 h (D). *p < 0.05 vs LPS. Data shown are representative of three different experiments. (E-F) LPS-stimulated RAW264.7 cells (1 × 106/ml) were incubated with AS101 (2 μg/ml) for 24 h. The culture supernatants were subsequently isolated and analyzed for nitrite and IL-6 levels. Data expressed as mean ± SE of four independent experiments. ** p < 0.05 vs. control, * p < 0.05 vs. LPS.