Pyk2 expression in HL60 cells differentiated by DMSO. A) HL60 cells were induced to differentiate toward neutrophil-like cells. HL60 cells were lysed before and 3 to 5 days after differentiation, and Pyk2 expression in these cells was analyzed by Western blot using anti-Pyk2 antibody. B) Pyk2 expression in dHL60 cells as compared with human blood neutrophils. Pyk2 expression was measured as above. Representative blot is shown (n = 3).