Severe local thermal injury markedly heightens the remote ear-hole inflammatory processes resulting in attenuated tissue healing. MRL/MpJ mice with ear-hole wounds ± thermal injury were evaluated. Gene transcript levels obtained from tissue collected at the edge/margin of the ear-hole wounds were assessed using quantitative RT-PCR as described in the Material and Methods. The expression of transcripts normalized to 18 s in ear tissue at 6 and 24 hrs post thermal injury were determined. Each value represents the mean ± SEM versus ear tissue from uninjured control MRL/MpJ mice (n = 5-7 mice/time point, * P < 0.05 as compared with baseline time 0-hr levels).