Table 1 Details regarding antibodies used
From: Proposed protective mechanism of the pancreas in the rat
Primary antibody (dilution, manufacturer) | Secondary antibody | Visualization |
|---|---|---|
CINC-1 (1:10, R&D Systems) | Biotinylated anti-Gt ABC Vectastain | DAB |
Desmin (1:400, Sigma) | Biotinylated anti-Ms ABC Vectastain | DAB |
ED-1 (1:400, Serotec) | Biotinylated anti-Ms ABC Vectastain | DAB |
MCP-1 (1:100, Abcam) | Biotinylated anti-Rb ABC Vectastain | DAB |
MPO (1:900, DakoCytomation) | Biotinylated anti-Rb ABC Vectastain | New Fuchsin |
α-SMA (1:200, Sigma) | * | New Fuchsin |