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Table 1 Details regarding antibodies used

From: Proposed protective mechanism of the pancreas in the rat

Primary antibody
(dilution, manufacturer)
Secondary antibody Visualization
CINC-1
(1:10, R&D Systems)
Biotinylated anti-Gt
ABC Vectastain
DAB
Desmin
(1:400, Sigma)
Biotinylated anti-Ms
ABC Vectastain
DAB
ED-1
(1:400, Serotec)
Biotinylated anti-Ms
ABC Vectastain
DAB
MCP-1
(1:100, Abcam)
Biotinylated anti-Rb
ABC Vectastain
DAB
MPO
(1:900, DakoCytomation)
Biotinylated anti-Rb
ABC Vectastain
New Fuchsin
α-SMA
(1:200, Sigma)
* New Fuchsin
  1. cytokine-induced neutrophil chemoattractant-1 (CINC-1), monocyte chemotactic protein-1 (MCP-1), myeloperoxidase (MPO), α-smooth muscle actin (α-SMA), * alkaline phosphatase-conjugated primary antibody, no secondary antibody used.