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Figure 7 | Journal of Inflammation

Figure 7

From: Differential splicing of the apoptosis-associated speck like protein containing a caspase recruitment domain (ASC) regulates inflammasomes

Figure 7

ASC and ASC-b interact with pro-caspase 1 with similar affinity. Immobilized GST-caspase 1-CARD was incubated with in vitro translated and biotinylated ASC or ASC-b and subjected to in vitro GST-pull down assays using GST-caspase 1-CARD and GST control immobilized to GSH Sepharose, as indicated. Bound proteins were visualized with streptavidin-HRP and ECL-Plus detection (Amersham Pharmacia Biotech). 10% of the in vitro translated proteins were loaded as 'input".

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Journal of Inflammation

ISSN: 1476-9255

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