Localization of ASC isoforms. Subcellular localization of the myc-tagged ASC isoforms was examined in transiently transfected HEK293 cells. Cells were fixed and immunostained with monoclonal anti-myc antibodies and Alexa Fluor 488-conjugatetd secondary antibodies. Nuclei and actin were visualized using Topro-3 and Alexa Fluor 546-conjugated phalloidin, respectively. Images were acquired by laser scanning confocal microscopy, showing from left to right ASC (green), nucleus (blue), actin (red) and a merged composite image. The panels show ASC (1st and 2nd panels), ASC-b (3rd panel), ASC-c (4th panel), ASC-d (5th panel), and vector control (6th panel).