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Figure 2 | Journal of Inflammation

Figure 2

From: Differential splicing of the apoptosis-associated speck like protein containing a caspase recruitment domain (ASC) regulates inflammasomes

Figure 2

Three novel ASC isoforms. (A) Clustal W alignment of ASC, ASC-b, ASC-c and ASC-d. ASC consists of a PYD, linker, and CARD, while ASC-b displays an in frame deletion of amino acids 93 to 111, corresponding to the complete linker region. ASC-c lacks amino acids 26 to 85 corresponding to helices 3 to 6 of the ASC-PYD, and in ASC-d amino acids 36-195 are replaced with 69 unrelated amino acids due to a frame shift resulting in the deletion of nucleotides 107 to 134 in ASC-d. (B) Schemata showing the domain structure of the ASC isoforms. (C) Myc-tagged ASC, ASC-b, ASC-c and ASC-d were transiently transfected into HEK293 cells and expression of ASC proteins with the predicted molecular weight was verified by immunoblot using anti-myc antibodies.

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Journal of Inflammation

ISSN: 1476-9255

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