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Figure 6 | Journal of Inflammation

Figure 6

From: Cytosolic group IVa phospholipase A2 mediates IL-8/CXCL8-induced transmigration of human polymorphonuclear leukocytes in vitro

Figure 6

Change in cell shape and F-actin polymerization of treated PMNs. (a-d) At 90 min incubation time, migrated PMNs (N = 4 donors) were harvested from the IL-8/CXCL8 chamber of the transwell migration apparatus. Change in cell shape was examined by confocal microscopy. Resting PMNs treated with either buffer (a; baseline), 30 ÎĽM TFMK (c) or (d) 10-6 M pyrrophenone (pyrro) for 30 min prior to migration toward IL-8/CXCL8. Treated PMNs were then fixed with 10% paraformaldehyde solution and cytoslides were prepared to examine the change in cell shape. For all groups, baseline is buffer-treated cells. Photomicrographs depict high power (630Ă— magnification). (e-h) Representative confocal photomicrographs of F-actin polymerization caused by buffer (e) or IL-8/CXCL8 (f) in the presence or absence of arachidonyl trifluoromethylketone (TFMK; g) or pyrrophenone (pyrro; h). For all groups, baseline is buffer-treated cells (e). Photomicrographs depict high power (630Ă— magnification).

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