Figure 3
Phosphorylation (phos) of group IVa phospholipase A 2 (gIVaPLA 2 ) caused by IL-8/CXCL8 activation. a) Treated PMNs were activated with 100 ng/ml IL-8/CXCL8 at different time intervals, in minutes (min) and were lysed immediately. Treated samples were subjected to Western blot analysis and probed with pAb directed against Ser505 gIVaPLA2 (top) or pAb against total gIVaPLA2 (bottom). Total gIVaPLA2 protein was stained with pAb directed against total gIVaPLA2 to indicate equal loading of samples. Representative immunoblot from 3 independent experiments. b) Translocation of cytosolic gIVaPLA2 to the nuclear component of PMNs was examined by subfractionation. Representative blot from 3 different donors was shown after staining with mAb against gIVaPLA2.