Figure 2

Phosphorylation (phos) of ERK-1/2, p38 MAPK, and Akt/PKB caused by IL-8/CXCL8. Whole cells extracts were prepared from isolated PMN treated with 100 ng/ml IL-8/CXCL8 at different times. Lysed PMNs were then stained with specific pAb directed against phosphorylated a) ERK-1/2, b) p38 MAPK or c) Akt/PKB. Equal loading of sample is demonstrated by staining of total protein for each kinase. Results shown are representative immunoblots obtained from 4 healthy donors. Negative control (-con) is buffer-activated cells.