Figure 6

Semi-quantitative analysis of apoptosis in DNCB-induced rashes treated for 24 h with nanocrystalline silver-derived solutions. Semi-quantitative analysis of apoptotic staining in biopsies from pigs with DNCB-induced rashes after 24 h of various treatments is shown. The relative apoptotic staining level was calculated by taking a ratio of apoptotic staining (where colocalized with nuclear staining) to total nuclear staining in a given image window. Semi-quantitative analysis of staining in the epidermis, superficial dermis, deep dermis, and all dermal images combined are shown in (A) through (D), respectively. Statistical analysis, which was performed using Kruskal Wallis testing with Dunn's Multiple Comparisons post testing, is provided in Table 3. Error bars represent standard deviations.