VEGFR2-PI-3K association in rat lungs exposed to CS. (A) A representative immunoblot picture of immunoprecipitated VEGFR2 probed for the p85 catalytic subunit of PI-3K after 3 days, 8 weeks and 6 months of CS exposure in rat lungs. The interaction of the p85 subunit of PI-3K was unaltered after 3 days and 8 weeks of CS exposure but was significantly increased after 6 months of CS exposure compared to sham-exposed animals (n = 6). (B) Histograms represent the Mean ± SE of percentage of VEGFR2/PI-3K association. ** p < 0.01 compared to sham-exposed animals.