Figure 4

PI3Kinase plays a role in LPS induced TNFalpha secretion. Panel A1: Effect of PI3K inhibitor wortmannin and PKC inhibitor on TNFalpha protein secretion. Concentrations of TNFalpha (pg/mL) were determined at 6 hours in the medium of mature adipocyte cultures treated or not with: 1 μg/mL of LPS alone; LPS and PI3K inhibitor (wortmannin, 1 and 5 μM); LPS and PKC inhibitor (chelerythrine, 1 μM); LPS and wortmannin + chelerythirine. The graph represents the mean ± SE of the results from one patient (n = 6 for each condition), representative of four experiments on four different patients. ***P < 0.001%, vs. control cells; §§§SP < 0.001%, vs. LPS + wort1 treated cells; ###P < 0.001%, vs. LPS + wort5 treated cells. Panel A2: Effect of PI3K specific inhibitor LY294002 on TNFalpha protein secretion. Mature adipocytes were treated with LPS (1 μg/mL) and/or not with PI3K specific inhibitor, LY294002 (100 nM and 500 nM). The concentration of TNFalpha (pg/mL) was determined in medium after 6 hours treatment. The graph represents the mean of the results from one patient (n = 6 for each condition), representative of three experiments on three different patients. **P < 0.005%, *P < 0.01%, vs. LPS treated cells. Panel B: Effect of PI3K inhibitors wortmannin on TNFalpha gene expression. TNFalpha gene expression in mature adipocyte cultures, treated or not with: 1 μg/mL of LPS alone, or LPS and PI3K inhibitor (wortmannin, 1 μM) were determined at 3, 4, 5 and 6 hours of treatment. The graph represents the mean ± SD of the results from 2 patients (n = 5 for each condition, for each patient). ***P < 0.001%, *P < 0.05% vs. control cells.