Figure 3

LPS induced TNFalpha protein secretion is independent of MAP Kinase Kinase, c-jun MAP kinase and protein kinase A pathways. Panel A: Effect of Raf-1, MEK1/2 and c-jun kinase inhibitors. Concentrations of TNFalpha (pg/mL) were determined at 6 hours in the medium of mature adipocyte cultures treated or not with: 1 μg/mL of LPS alone; LPS and Raf-1 specific inhibitor (GW5074, 5 μM); LPS and MEK1/2 inhibitor (U0126, 1 μM); LPS and c-jun kinase inhibitor (JNKi, 1 μM). The graph represents the mean ± SE of the results from two patients (n = 6 for each condition), representative of four experiments on four different patients. Panel B: Effect of protein kinase A inhibitor. Concentrations of TNFalpha (pg/mL) were determined at 6 hours in the medium of mature adipocyte cultures treated or not with 1 μg/mL of LPS alone or LPS and increasing concentrations of PKA specific inhibitor (PKAi, 20, 40 and 80 nM). The graph represents the mean ± SE of the results from two patients (n = 6 for each condition).