p38 MAP Kinase phosphorylation by LPS. Mature adipocyte cells were treated with LPS at 1 μg/mL for 5, 10 and 20 min, or with LPS + p38 MAP Kinase inhibitor (SB, 1 μM) for 5 min. Proteins (50 μg per lane) were separated by SDS-PAGE and analyzed by Western blotting using an anti-phospho-p38 MAP Kinase protein antibody (Thr180/Tyr182, panel B). Loading equality was controlled using antibody against the unphosphorylated isoform of p38 (panel A). The data represent a typical result from two independent experiments.